Research Profile for Spyridonidis Alexandros


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General
Bone Marrow Transplantation Laboratory
Department of Medicine
Research Activity
bone marrow transplantation, stem cells, leukemia, graft versus host disease, genomic instability, adoptive cell therapy
Research Area of Interest
- Hematopoietic Stem Cells and Engineering, Adult Stem cells (Ex vivo expansion, purging, stem cell plasticity)
- Leukemia (Chemoresistance mechanisms in Leukemia, Leukemic Stem Cells)
- Hematopoietic Cell Transplantation (Pathogenesis of GvHD, Allo-Immunoregulation, adoptive cell therapy as translational research)

Clinical Area of Interest
- Haematological Malignancies and especially Leukaemia’s
- Hematopoietic Cell Transplantation
Research Infrastructure
Principal Investigator
Alexandros Spyridonidis, MD, PhD
Ass. Professor Hematology

Lagadinou Eleni, MD/PHD/Post doc
Maria Themeli, MD PhD candidate
Lazana Ioanna, MD PhD candidate
Kokkinou Dimitra, Biologist PhD candidate
Zoudiari Anastasia, Biologist PhD candidate
Georgia Oikonomopoulou Technician
- Standard equipment for culture work
- Standard equipment for molecular studies (including real time PCR), protein biochemistry and genetic engineering
- FACS Analyzer and FACS-Sorter (Vantage SE, Becton Dickinson
- Microsopes including fluorescent microscopes
- A GMP Facility is currently under cosntruction

Also access to the Core Facility equipments of the Medical School of Patras
- Confocal Microscope (Leica SP5) equiped for live-cell imaging, time-lapse, FRAP, FRET, FLIP
- Microarray Hybridizer and Scanner
- State-of-the-art animal facility
- Capillary Electrophoresis ABI Prism Genetic Analyzer
Research Activities
- Graft versus host Disease Biology (Themeli)
- Stem cell plasticity (Themeli)
- Mechanisms of chemoresistance in Leukemia (Lagadinou)
- Leukemic cell biology and targeting (Lagadinou, Kokkinou)
- Adoptive cell therapy after transplantation (translational research) (Lazana, Zoudiari)

Academic (institutional) clinical research
- GvHD protocols (BMT 2010, in press)
- NK cell therapy for leukemia treatment (TaNK Study)

Funding:
- Greek Ministry of Education, Program Irakleitos II 2007-2013 (45.000 Euro)
- Greek Ministry of Health, 2008-2011 (12.000 Euro)
- State Scholarships Foundation, Greece (IKY), 2007-2009 (15.000 Euro)
- Desmi 2008 (European Union and rep. of Cyprus), Collaborator for the development of the Center of the Study for Haematological Malignancies in Cyprus, 2009-2012
- The General Secretariat for Research and Technology of the Ministry of Development 2006-2008 (12.000 Euro)
- Landesstiftung Baden-Württemberg gGmbH 2003-2008(630.000 Euro)
- Biothera Stiftung, Germany 2000-2005 (30.000 Euro)
- A. Spyridonidis, M. Liga, E. Triantafyllou, M. Themeli, M. Karakantza, N. Zoumbos. Pharmacokinetics and clinical activity of very low dose alemtuzumab in transplantation for acute leukemia Bone Marrow Transplant (in press, 2010)
- Waterhouse M, Themeli M, Bertz H, Zoumbos N, Finke J, Spyridonidis A. Horizontal DNA transfer from donor to host cells as an alternative mechanism of epithelial chimerism after allogeneic hematopoietic cell transplantation. Biol Blood Marrow Transplant. (in press, 2010).
- M Themeli, L Petrikkos, M Waterhouse, H Bertz, E Lagadinou, N Zoumbos, J Finke and A Spyridonidis. Alloreactive microenvironment after human hematopoietic cell transplantation induces genomic alterations in epithelium through an ROS-mediated mechanism: in vivo and in vitro study and implications to secondary neoplasia. Leukemia 24, 536-543, 2010.
- E. Lagadinou, P. Ziros, O. Tsopra, K. Dimas, D. Kokkinou, E. Thanopoulou, M. Karakantza, P. Pantazis, A. Spyridonidis, N. Zoumbos. C-Jun N-terminal kinase activation failure is a new mechanism of anthracycline resistance in acute myelod leukemia. Leukemia 10, 1899-908, 2008.
- Marks R, Potthoff K, Hahn J, Ihorst G, Bertz H, Spyridonidis A, Holler E, Finke JM. Reduced-toxicity conditioning with fludarabine, BCNU, and melphalan in allogeneic hematopoietic cell transplantation: particular activity against advanced hematologic malignancies. Blood 112(2):415-25, 2008.
- Faber P, Fisch P, Waterhouse M, Schmitt-Graff A, Bertz H, Finke J, Spyridonidis A. Frequent genomic alterations in epithelium measured by microsatellite instability following allogeneic hematopoietic cell transplantation in humans. Blood 5:107:3389-3396, 2006.
- Metaxas Y, Zeiser R, Schmitt-Graeff A, Waterhouse M, Faber P, Follo M, BertzH, Finke J, Spyridonidis A. Human hematopoietic cell transplantation results in generation of donor-derived epithelial cells. Leukemia. 19(7):1287-9, 2005
- Spyridonidis A, Bertz H, Ihorst G, Grullich C, Finke J. Hematopoietic cell transplantation from unrelated donors as an effectivetherapy for older patients (> or = 60 years) with active myeloid malignancies. Blood. 105(10):4147-8, 2005
-Spyridonidis A, Dwenger A, Tomann T, Follo M, Behringer D, Finke J. Epithelial tissue chimerism after human hematopoietic cell transplantation is a real phenomenon. Am J Pathol 164:1147-1155, 2004
- Dwenger A, Rosenthal F, Machein M, Waller C, Spyridonidis A Transplanted bone marrow cells preferentially home to the vessels of in situ generated murine tumors rather than of normal organs. Stem cells 22: 86-92, 2004
- Spyridonidis A, Bernhardt W, Behringer D, Köhler G, Azemar A, Pflug A, Henschler R: Influence of culture conditions on the survival and proliferation of tumor cells during ex vivo culture of CD34+ progenitor cells. Blood 93:746-755, 1999
- Spyridonidis A, Schmidt M, Bernhardt W, Papadimitriou A, Azemar M, Wels W, Groner B, Henschler R: Purging of mammary carcinoma cells during ex vivo culture of CD34+ hematopoietic progenitors with recombinant immunotoxins. Blood 91:1820-1827, 1998
Internal Cooperations:
Participating in following research networks of U of Patras
- PHARMOGLOBAL (URL under construction)
- HYGEIA (URL under construction)

External Cooperations:
- U Freiburg, Germany, BMT Laboratory / Prof. Dr. J. Finke and Immunoregulation Lab / PD Dr Zeiser, (http://www.uniklinik freiburg.de/medizin1/live/forschung)
- Immunogenetics Center (Karaiskakio Foundation) and Center of the Study for Haematological Malignancies, Cyprus, Dr. Paul Costeas (http://www.karaiskakio.org.cy/v1_en/front-end/main.php?action=aatext&page=&design=default&aatext_id=2)
- U Thessaloniki, Greece, Dept of Pharmaceutical Sciences, Prof. Tsiftsoglou (http://www.pharm.auth.gr/gr/profiles/tsiftsoglou.html)
- Academy of Athens, Cell and Gene Therapy Lab, Vassilopoulos George, (http://www.bioacademy.gr/Faculty/investigators.php?st=1)
- U Thrace / Alexandroupolis, Greece, Hematology Division, Kotsianidis I, (http://www.med.duth.gr/index.php?option=com_content&task=view&id=58&Itemid)
-U Thessaly / Larisa, Greece, Hematology Div. Prof. Matsouka, Dr. Giannakoulas
Additional Information
The Laboratory performs routinely following analyses which support the clinical activities of the University Hospital:
- Microsattelite PCR for chimerism analyses (daily)
- Cyclosporine levels (daily)
- HLA A* ,B* , DR* (SSP, two digit molecular analysis) (results within one week)

Other services:
- FACS sorting
- Isolation of cell subsets with MACS

For information regarding these services please contact per email or tel +30 2610 969110